PIPs: Human Protein-Protein Interaction Prediction

Evidence of Interaction Between RPA1 and MCM2

The interaction score between RPA1 and MCM2 is 1.59E4.

This page provides the details about the evidence that was used to calculate the interaction Score between the two proteins

The Scores that are listed with each module are based on the interaction score where that module provides the only source of evidence. The final Score is the integration of all the sources of evidence.

Feature

Evidence

Score (?)

Gene Expression

Pearson's Correlation between RPA1 and MCM2 = 0.80

The original expression data was collated from the NCBI GEO database. For further information about this dataset please refer to the NCBI GEO dataset GDS596.

0.027

Orthology

Organism	RPA1 Orthologue		MCM2 Orthologue		Experiment
Organism	Accession	Inparalog Score	Accession	Inparalog Score	Experiment
bakers yeast	YAR007C	1	YBL023C	1	AffinityCaptureWestern Get References From PubMed
worm	Q19537	1	Q9XXI9	1
fly	Q24492	1	P49735	1

The Inparalog Score is calculated by InParanoid

The experiment type Other refers to techniques other than Y2H, MS, Immunoprecipitation, SurfacePlasmonResonance, XrayDiffraction, Xlinking, CompetitionBinding, Immunofluorescence, GelRetardationAssay, AffinityChromatography, InVitroBinding, InVitro, InVivo, FarWestern, pullDown, AffinityCaptureMS, EpistaticMiniArrayProfile, AffinityCaptureWestern, BiochemicalActivity, CoCrystalStructure, FRET, ReconstitutedComplex, CoLocalization, CoPurification or CoFractionation.

0.593

Domains

Domains present in RPA1 and MCM2:

Domains in RPA1

Domains in MCM2

IPR008994	Nucleic acid-binding OB-fold
IPR004365	OB-fold nucleic acid binding
IPR004591	Replication factor-a protein 1 Rpa1
IPR007199	Replication factor-A protein 1, N-terminal
IPR012340	OB_NA_bd_sub.

IPR008994	Nucleic acid-binding OB-fold
IPR001208	MCM
IPR008045	MCM protein 2

The Chi square scores for co-occurrence of domains that are present in RPA1 and MCM2 are listed below.

Domain 1		Domain 2		Chi Square
IPR008994	Nucleic acid-binding OB-fold	IPR001208	MCM	301.459
IPR008994	Nucleic acid-binding OB-fold	IPR008994	Nucleic acid-binding OB-fold	191.222
IPR012340	OB_NA_bd_sub.	IPR008994	Nucleic acid-binding OB-fold	168.682
IPR012340	OB_NA_bd_sub.	IPR008045	MCM protein 2	83.8537
IPR004365	OB-fold nucleic acid binding	IPR001208	MCM	56.6924
IPR008994	Nucleic acid-binding OB-fold	IPR008045	MCM protein 2	51.0284
IPR012340	OB_NA_bd_sub.	IPR001208	MCM	49.9995
IPR004365	OB-fold nucleic acid binding	IPR008994	Nucleic acid-binding OB-fold	29.2304

The background colour of the table refers to:

Blue Domain is only present in RPA1
Yellow Domain is only present in MCM2
Green Domain is present in both RPA1 and MCM2

0.027

Post-translational Modifications

Protein: RPA1

PTM	Residue	Reference

Protein: MCM2

PTM	Residue	Reference
Phosphorylation	26	PubMed, HPRD
Phosphorylation	27	PubMed, HPRD
Phosphorylation	41	PubMed, HPRD
Phosphorylation	106	PubMed, HPRD
Phosphorylation	139	PubMed, HPRD
Phosphoserine	27	HPRD
Phosphoserine	41	HPRD
Phosphoserine	108	PubMed, HPRD
Phosphothreonine	106	HPRD

Localisation

Protein: RPA1

Localisation	Reference
Nucleus	HPRD
Cytoplasm	HPRD
Cytoskeleton	HPRD

Protein: MCM2

Localisation	Reference
Nucleus	HPRD
Nucleoplasm	HPRD

Transitive Score

The common interactors between RPA1 and MCM2 that are considered by the Transitive module are listed below.

The Scores listed are the values used by the transitive module to calculate the final interaction Score between RPA1 andMCM2. The values listed are therefore not the final Score value for the listed interactions.

With a Score value ≥0.025 , RPA1 has 291 interactors and MCM2 has 983 interactors of which there are 157 common interactors.

Common Interactor	Name of Common Interactor	Score for RPA1-Interactor (?)	Score for MCM2-Interactor (?)
RPA2	RPA2, REPA2, RPA32, RPA34: Replication protein A 32 kDa subunit	80.90	0.225
MCM5	MCM5, CDC46: DNA replication licensing factor MCM5	69.60	209.00
PSMC1	PSMC1: 26S protease regulatory subunit 4	25.60	0.304
RUVBL1	RUVBL1, NMP238, TIP49, TIP49A: RuvB-like 1	12.50	0.047
RAD51	RAD51, RAD51A, RECA: DNA repair protein RAD51 homolog 1	6.33	0.148
ATR	ATR, FRP1: Serine/threonine-protein kinase ATR	3.99	0.046
POLR2A	POLR2A: Uncharacterized protein POLR2A	2.95	0.114
MSH6	MSH6, GTBP: DNA mismatch repair protein MSH6	1.82	0.129
H3F3A	H3F3A, H3.3A, PP781, H3.3B, H3F3B, H3F3: Histone H3.3	1.10	0.046
CORO1C	CORO1C, CRNN4: Coronin-1C	0.890	0.046
MRE11A	MRE11A, HNGS1, MRE11: Double-strand break repair protein MRE11A	0.890	0.063
HMGB2	HMGB2, HMG2: High mobility group protein B2	0.782	0.827
DHX15	DHX15, DBP1, DDX15: Putative pre-mRNA-splicing factor ATP-dependent RNA helicase	0.293	0.137
KIAA0788	ASCC3L1, HELIC2, KIAA0788: U5 small nuclear ribonucleoprotein 200 kDa helicase	0.263	0.233
EIF2S1	EIF2S1, EIF2A: Eukaryotic translation initiation factor 2 subunit 1	0.263	0.763
MCM3	MCM3: DNA replication licensing factor MCM3	0.263	1.29E3
SFRS1	SFRS1, ASF, OK/SW-cl.3, SF2, SF2P33: Splicing factor, arginine/serine-rich 1	0.242	0.515
EXOSC8	EXOSC8, OIP2, RRP43: Exosome complex exonuclease RRP43	0.231	0.108
KARS	KARS, KIAA0070: Lysyl-tRNA synthetase	0.231	0.065
PSMC3	PSMC3, TBP1: 26S protease regulatory subunit 6A	0.207	0.0968
SRP54	SRP54: Signal recognition particle 54 kDa protein	0.207	0.059
CDC45L	CDC45L, CDC45L2, UNQ374/PRO710: CDC45-related protein	0.196	0.196
CHD4	CHD4: Chromodomain-helicase-DNA-binding protein 4	0.137	0.260
DDX21	DDX21: Nucleolar RNA helicase 2	0.137	0.260
DDX46	DDX46, KIAA0801: Probable ATP-dependent RNA helicase DDX46	0.137	0.260
MCM7	MCM7, CDC47, MCM2: DNA replication licensing factor MCM7	0.123	209.00
BTAF1	BTAF1, TAF172: TATA-binding protein-associated factor 172	0.123	0.188
RECQL	RECQL, RECQL1: ATP-dependent DNA helicase Q1	0.123	0.044
KHDRBS1	KHDRBS1, SAM68: KH domain-containing, RNA-binding, signal transduction-associate	0.123	0.233
DDX1	DDX1: ATP-dependent RNA helicase DDX1	0.123	0.038
SMARCA5	SMARCA5, SNF2H, WCRF135: SWI/SNF-related matrix-associated actin-dependent regul	0.123	0.233
ORC2L	ORC2L, ORC2: Origin recognition complex subunit 2	0.123	0.123
EIF2S2	EIF2S2, EIF2B: Eukaryotic translation initiation factor 2 subunit 2	0.123	0.461
YBX1	YBX1, NSEP1, YB1: Nuclease sensitive element-binding protein 1	0.123	0.461
DDX3X	DDX3X, DBX, DDX3: ATP-dependent RNA helicase DDX3X	0.123	0.139
ABCE1	ABCE1, RLI, OK/SW-cl.40, RNASEL1, RNASELI, RNS4I: ATP-binding cassette sub-famil	0.123	0.123
MCM6	MCM6: DNA replication licensing factor MCM6	0.123	69.60
MCM4	MCM4, CDC21: DNA replication licensing factor MCM4	0.123	1.29E3
ASCC3	ASCC3, HELIC1: Activating signal cointegrator 1 complex subunit 3	0.123	0.074
MRPS28	MRPS28, MRPS35, HSPC007: Mitochondrial 28S ribosomal protein S28	0.123	0.123
SCYE1	SCYE1, EMAP2: Multisynthetase complex auxiliary component p43 [Contains: Endothe	0.123	0.060
POLR2H	POLR2H: DNA-directed RNA polymerases I, II, and III subunit RPABC3	0.123	0.156
RPS23	RPS23: 40S ribosomal protein S23	0.123	0.044
FUBP1	N/A	0.108	0.124
HIST1H3A	HIST1H3A, H3FA, HIST1H3B, H3FL, HIST1H3C, H3FC, HIST1H3D, H3FB, HIST1H3E, H3FD,	0.108	0.029
PSMC6	PSMC6, SUG2: 26S protease regulatory subunit S10B	0.0968	0.059
PCBP1	PCBP1: Poly(rC)-binding protein 1	0.0968	0.111
RFC4	RFC4: Replication factor C subunit 4	0.0968	0.207
RFC5	RFC5: Replication factor C subunit 5	0.0968	0.207
EXOSC7	EXOSC7, KIAA0116, RRP42: Exosome complex exonuclease RRP42	0.0968	0.059
RFC3	RFC3: Replication factor C subunit 3	0.0968	0.207
PSMC2	PSMC2, MSS1: 26S protease regulatory subunit 7	0.0968	0.304
DARS	DARS, PIG40: Aspartyl-tRNA synthetase, cytoplasmic	0.0968	0.059
EIF2S3	EIF2S3, EIF2G: Eukaryotic translation initiation factor 2 subunit 3	0.0968	0.032
HRB2	KRR1, HRB2: KRR1 small subunit processome component homolog	0.0968	0.032
RBMX	RBMX, HNRPG, RBMXP1: Heterogeneous nuclear ribonucleoprotein G	0.090	0.269
POT1	POT1: Protection of telomeres protein 1	0.083	0.067
MUTYH	N/A	0.083	0.083
SNRPA	SNRPA: U1 small nuclear ribonucleoprotein A	0.081	0.065
SFRS10	SFRS10, TRA2B: Splicing factor, arginine/serine-rich 10	0.081	0.241
SFRS4	SFRS4, SRP75: Splicing factor, arginine/serine-rich 4	0.081	0.146
HNRPF	HNRPF: Heterogeneous nuclear ribonucleoprotein F	0.081	0.241
SYNCRIP	SYNCRIP, HNRPQ, NSAP1: Heterogeneous nuclear ribonucleoprotein Q	0.081	0.241
TIAL1	TIAL1: Nucleolysin TIAR	0.081	0.146
SFRS2	SFRS2: Splicing factor, arginine/serine-rich 2	0.081	0.241
DDX41	DDX41, ABS: Probable ATP-dependent RNA helicase DDX41	0.074	0.139
DDX5	DDX5, G17P1, HELR, HLR1: Probable ATP-dependent RNA helicase DDX5	0.074	0.114
POLR2G	POLR2G: DNA-directed RNA polymerase II subunit RPB7	0.074	0.044
DHX16	DHX16, DBP2, DDX16, KIAA0577: Putative pre-mRNA-splicing factor ATP-dependent RN	0.074	0.114
ORC6L	ORC6L: Origin recognition complex subunit 6	0.074	0.123
YARS	YARS: Tyrosyl-tRNA synthetase, cytoplasmic	0.074	0.372
EIF1AX	EIF1AX, EIF1A, EIF4C: Eukaryotic translation initiation factor 1A, X-chromosomal	0.074	0.060
EIF2B5	EIF2B5, EIF2BE: Translation initiation factor eIF-2B subunit epsilon	0.074	0.372
RAD54L	RAD54L, RAD54A: DNA repair and recombination protein RAD54-like	0.074	0.081
HELLS	HELLS, PASG, Nbla10143, SMARCA6: Lymphoid-specific helicase	0.074	0.081
GCLC	GCLC, GLCL, GLCLC: Glutamate--cysteine ligase catalytic subunit	0.074	0.060
CDC6	CDC6, CDC18L: Cell division control protein 6 homolog	0.074	431.00
HAT1	HAT1: Histone acetyltransferase type B catalytic subunit	0.0739	6.52
OBFC1	OBFC1: Oligonucleotide/oligosaccharide-binding fold-containing protein 1	0.067	0.041
SMARCA4	SMARCA4, BRG1, SNF2B, SNF2L4: Probable global transcription activator SNF2L4	0.067	0.210
RAD54B	RAD54B: DNA repair and recombination protein RAD54B	0.067	0.0255
EIF5AP1	EIF5A: Eukaryotic translation initiation factor 5A-1	0.067	0.307
SRPR	SRPR: Uncharacterized protein SRPR	0.065	0.166
DICER1	DICER1, DICER, HERNA, KIAA0928: Endoribonuclease Dicer	0.060	0.114
BLM	BLM, RECQ2, RECQL3: Bloom syndrome protein	0.060	0.763
ORC1L	ORC1L, ORC1, PARC1: Origin recognition complex subunit 1	0.060	0.763
WRN	WRN, RECQ3, RECQL2: Werner syndrome ATP-dependent helicase	0.060	0.763
MDN1	MDN1, KIAA0301: Midasin	0.060	0.275
RECQL4	RECQL4, RECQ4: ATP-dependent DNA helicase Q4	0.060	0.123
SKIV2L	SKIV2L, XXbac-BCX116I9.5-001, XXbac-BPG116M5.1-001: Superkiller viralicidic acti	0.060	0.114
EIF1AY	EIF1AY: Eukaryotic translation initiation factor 1A, Y-chromosomal	0.060	0.060
NARS	NARS, ASNS: Asparaginyl-tRNA synthetase, cytoplasmic	0.059	0.035
PCBP2	PCBP2: Poly(rC)-binding protein 2	0.059	0.0818
ATAD2	ATAD2, L16, PRO2000: ATPase family AAA domain-containing protein 2	0.059	0.503
FXR1	FXR1: Fragile X mental retardation syndrome-related protein 1	0.059	0.245
VCP	VCP: Transitional endoplasmic reticulum ATPase	0.059	0.245
EIF5B	EIF5B, IF2, KIAA0741: Eukaryotic translation initiation factor 5B	0.059	0.040
ATAD3A	ATAD3A: ATPase family AAA domain-containing protein 3A	0.059	0.0968
SSBP1	SSBP1, SSBP: Single-stranded DNA-binding protein, mitochondrial precursor	0.055	0.0331
XAB1	XAB1, MBDIN, HUSSY-23: XPA-binding protein 1	0.055	0.027
TP53	TP53, P53: Cellular tumor antigen p53	0.053	0.241
GTF2B	GTF2B, TF2B, TFIIB: Transcription initiation factor IIB	0.053	0.372
G3BP	G3BP1, G3BP: Ras GTPase-activating protein-binding protein 1	0.051	0.185
BRCA2	BRCA2, FACD, FANCD1: Breast cancer type 2 susceptibility protein	0.050	0.515
VPS4A	VPS4A, VPS4: Vacuolar protein sorting-associating protein 4A	0.047	0.029
TRIP13	TRIP13: Thyroid receptor-interacting protein 13	0.047	0.304
DDX24	DDX24: ATP-dependent RNA helicase DDX24	0.044	0.086
EXOSC2	EXOSC2, RRP4: Exosome complex exonuclease RRP4	0.044	0.275
DDX3Y	DDX3Y, DBY: ATP-dependent RNA helicase DDX3Y	0.044	0.086
ERCC3	ERCC3, XPB, XPBC: TFIIH basal transcription factor complex helicase XPB subunit	0.044	0.114
SMARCA3	HLTF, HIP116A, RNF80, SMARCA3, SNF2L3, ZBU1: Helicase-like transcription factor	0.044	0.188
XRCC3	XRCC3: DNA-repair protein XRCC3	0.044	0.074
MATR3	MATR3, KIAA0723: Matrin-3	0.042	0.132
RBM3	RBM3, RNPL: Putative RNA-binding protein 3	0.042	0.0971
RBM14	RBM14, SIP: RNA-binding protein 14	0.042	0.163
CCNF	CCNF: G2/mitotic-specific cyclin-F	0.042	0.293
RFX5	RFX5: DNA-binding protein RFX5	0.042	0.067
ADAR	ADAR, ADAR1, DSRAD, G1P1, IFI4: Double-stranded RNA-specific adenosine deaminase	0.042	0.041
PABPN1	PABPN1, PAB2, PABP2: Polyadenylate-binding protein 2	0.038	0.146
PABPC4	PABPC4, APP1, PABP4: Polyadenylate-binding protein 4	0.038	0.146
CCNB1	Cyclin B1	0.038	1.62
SSB	SSB: Lupus La protein	0.038	0.241
SAFB	SAFB, HAP, HET, SAFB1: Scaffold attachment factor B	0.038	0.118
U2AF1	U2AF1, U2AF35, U2AFBP: Splicing factor U2AF 35 kDa subunit	0.038	0.065
GTF2F1	GTF2F1, RAP74: Transcription initiation factor IIF subunit alpha	0.038	0.461
CCNB2	CCNB2: G2/mitotic-specific cyclin-B2	0.038	0.263
CCNA2	CCNA2, CCN1, CCNA: Cyclin-A2	0.038	0.263
ELAVL1	ELAVL1, HUR: ELAV-like protein 1	0.038	0.241
RBM15	RBM15, OTT: Putative RNA-binding protein 15	0.038	0.146
EIF3S9	EIF3S9: Eukaryotic translation initiation factor 3 subunit 9	0.038	0.241
SNRPB2	SNRPB2: U2 small nuclear ribonucleoprotein B''	0.038	0.146
CSDA	CSDA, DBPA: DNA-binding protein A	0.036	0.275
NTHL1	NTHL1, NTH1, OCTS3: Endonuclease III-like protein 1	0.036	0.036
RUVBL2	RUVBL2, TIP48, CGI-46, TIP49B: RuvB-like 2	0.035	0.035
EXOSC5	EXOSC5, CML28, RRP46: Exosome complex exonuclease RRP46	0.035	0.035
EXOSC4	EXOSC4, RRP41, SKI6: Exosome complex exonuclease RRP41	0.029	0.060
BCS1L	BCS1L, BCS1: Mitochondrial chaperone BCS1	0.029	0.029
NUSAP1	NUSAP1, ANKT, BM-037, PRO0310: Nucleolar and spindle-associated protein 1	0.027	0.027
SMC4L1	N/A	0.027	0.082
ZWILCH	Protein zwilch homolog.	0.027	0.027
TMPO	N/A	0.027	0.082
ZWINT	N/A	0.027	0.082
PTGES3	PTGES3, P23, TEBP: Prostaglandin E synthase 3	0.027	0.025
ABCC4	ABCC4, MRP4: Multidrug resistance-associated protein 4	0.027	0.027
PRSS15	LONP1, PRSS15: Lon protease homolog, mitochondrial precursor	0.027	0.027
RPA3	RPA3, REPA3, RPA14: Replication protein A 14 kDa subunit	0.027	0.027
ABCF2	ABCF2, HUSSY-18: ATP-binding cassette sub-family F member 2	0.027	0.027
SMARCAD1	SMARCAD1, KIAA1122: SWI/SNF-related matrix-associated actin-dependent regulator	0.027	0.083
SF1	SF1, ZFM1, ZNF162: Splicing factor 1	0.027	0.083
OGG1	N/A	0.027	0.027
ZCCHC17	ZCCHC17, PS1D, HSPC243, HSPC251, LDC4: Nucleolar protein of 40 kDa	0.027	0.027
DNAH11	N/A	0.027	0.027
RNPS1	RNPS1, LDC2: RNA-binding protein with serine-rich domain 1	0.0255	0.132
ZNF638	ZNF638, NP220, ZFML: Zinc finger protein 638	0.0255	0.070
ELF1	ELF1: ETS-related transcription factor Elf-1	0.0255	0.307
E2F1	E2F1, RBBP3: Transcription factor E2F1	0.0255	0.852
HNRPA0	HNRPA0: Heterogeneous nuclear ribonucleoprotein A0	0.0255	0.163

0.572

Total Score

1.59E4