PIPs: Human Protein-Protein Interaction Prediction

Evidence of Interaction Between CCNB2 and MCM3

The interaction score between CCNB2 and MCM3 is 1.59E4.

This page provides the details about the evidence that was used to calculate the interaction Score between the two proteins

The Scores that are listed with each module are based on the interaction score where that module provides the only source of evidence. The final Score is the integration of all the sources of evidence.

Feature

Evidence

Score (?)

Gene Expression

Pearson's Correlation between CCNB2 and MCM3 = 0.80

The original expression data was collated from the NCBI GEO database. For further information about this dataset please refer to the NCBI GEO dataset GDS596.

0.027

Orthology

Organism	CCNB2 Orthologue		MCM3 Orthologue		Experiment
Organism	Accession	Inparalog Score	Accession	Inparalog Score	Experiment
bakers yeast	YGR108W	0.396	YEL032W	1
bakers yeast	YPR119W	1	YEL032W	1	BiochemicalActivity Get References From PubMed
worm	Q10653	0.333	Q9XVR7	1
fly	P20439	0.246	P91675	1

The Inparalog Score is calculated by InParanoid

The experiment type Other refers to techniques other than Y2H, MS, Immunoprecipitation, SurfacePlasmonResonance, XrayDiffraction, Xlinking, CompetitionBinding, Immunofluorescence, GelRetardationAssay, AffinityChromatography, InVitroBinding, InVitro, InVivo, FarWestern, pullDown, AffinityCaptureMS, EpistaticMiniArrayProfile, AffinityCaptureWestern, BiochemicalActivity, CoCrystalStructure, FRET, ReconstitutedComplex, CoLocalization, CoPurification or CoFractionation.

0.593

Domains

Domains present in CCNB2 and MCM3:

Domains in CCNB2

Domains in MCM3

IPR011028	Cyclin-like
IPR006670	Cyclin
IPR006671	Cyclin, N-terminal
IPR004367	Cyclin, C-terminal
IPR013763	Cyclin-related

IPR003593	AAA ATPase
IPR008994	Nucleic acid-binding OB-fold
IPR001208	MCM
IPR008046	MCM protein 3

The Chi square scores for co-occurrence of domains that are present in CCNB2 and MCM3 are listed below.

Domain 1		Domain 2		Chi Square
IPR006670	Cyclin	IPR001208	MCM	15.4071
IPR011028	Cyclin-like	IPR001208	MCM	11.617
IPR013763	Cyclin-related	IPR001208	MCM	11.2935
IPR006670	Cyclin	IPR003593	AAA ATPase	0.931553
IPR011028	Cyclin-like	IPR003593	AAA ATPase	0.663281
IPR013763	Cyclin-related	IPR003593	AAA ATPase	0.604607

The background colour of the table refers to:

Blue Domain is only present in CCNB2
Yellow Domain is only present in MCM3
Green Domain is present in both CCNB2 and MCM3

0.027

Post-translational Modifications

Protein: CCNB2

PTM	Residue	Reference

Protein: MCM3

PTM	Residue	Reference
Phosphorylation	713	PubMed, HPRD
Phosphorylation	722	PubMed, HPRD
Phosphoserine	535	PubMed, HPRD
Phosphothreonine	713	HPRD
Phosphothreonine	722	HPRD

Localisation

Protein: CCNB2

Localisation	Reference
Golgi	HPRD
Secreted	HPRD
Cytoplasm	HPRD

Protein: MCM3

Localisation	Reference
Nucleus	HPRD
Nucleoplasm	HPRD

Transitive Score

The common interactors between CCNB2 and MCM3 that are considered by the Transitive module are listed below.

The Scores listed are the values used by the transitive module to calculate the final interaction Score between CCNB2 andMCM3. The values listed are therefore not the final Score value for the listed interactions.

With a Score value ≥0.025 , CCNB2 has 332 interactors and MCM3 has 1106 interactors of which there are 161 common interactors.

Common Interactor	Name of Common Interactor	Score for CCNB2-Interactor (?)	Score for MCM3-Interactor (?)
CDC6	CDC6, CDC18L: Cell division control protein 6 homolog	69.60	1.62
AURKA	AURKA, AIK, ARK1, AURA, BTAK, STK15, STK6: Serine/threonine-protein kinase 6	69.60	0.146
CDC25A	CDC25A: M-phase inducer phosphatase 1	32.20	0.025
AURKB	AURKB, AIK2, AIM1, ARK2, STK12: Serine/threonine-protein kinase 12	13.90	0.053
FOXO3A	FOXO3A, FKHRL1: Forkhead box protein O3A	11.70	0.171
CDK2	CDK2: Cell division protein kinase 2	6.55	27.40
CDC25C	CDC25C: M-phase inducer phosphatase 3	6.33	0.025
PDPK1	PDPK1, PDK1: 3-phosphoinositide-dependent protein kinase 1	6.33	0.047
ATAD2	ATAD2, L16, PRO2000: ATPase family AAA domain-containing protein 2	6.32	0.503
CDK3	CDK3: Cell division protein kinase 3	4.16	0.956
PRKCE	PRKCE, PKCE: Protein kinase C epsilon type	3.75	0.031
PSMC2	PSMC2, MSS1: 26S protease regulatory subunit 7	3.08	0.503
MSH6	MSH6, GTBP: DNA mismatch repair protein MSH6	1.82	0.827
ORC2L	ORC2L, ORC2: Origin recognition complex subunit 2	1.10	0.123
FOXO1A	FOXO1A, FKHR: Forkhead box protein O1A	0.837	0.111
PRKCG	PRKCG, PKCG: Protein kinase C gamma type	0.837	0.0308
CCNA2	CCNA2, CCN1, CCNA: Cyclin-A2	0.789	0.263
CCNB1	Cyclin B1	0.789	40.60
TTK	TTK, MPS1L1: Dual specificity protein kinase TTK	0.789	0.053
PRKCA	PRKCA, PKCA, PRKACA: Protein kinase C alpha type	0.754	0.035
RUNX1	N/A	0.741	0.046
PRKCQ	PRKCQ, PRKCT: Protein kinase C theta type	0.658	0.033
NAP1L1	NAP1L1, NRP: Nucleosome assembly protein 1-like 1	0.657	0.129
CDC23	CDC23: Cell division cycle protein 23 homolog	0.612	0.141
FZR1	N/A	0.538	0.038
DIAPH1	Diaphanous 1	0.408	0.046
USP10	USP10, KIAA0190: Ubiquitin carboxyl-terminal hydrolase 10	0.364	0.040
CAMKK1	N/A	0.354	0.025
SMC4L1	SMC4, CAPC, SMC4L1: Structural maintenance of chromosomes protein 4	0.354	0.075
FKBP1A	FKBP1A, FKBP1, FKBP12: FK506-binding protein 1A	0.326	0.029
DDX3Y	DDX3Y, DBY: ATP-dependent RNA helicase DDX3Y	0.326	0.114
CCNF	CCNF: G2/mitotic-specific cyclin-F	0.293	0.137
MCM6	MCM6: DNA replication licensing factor MCM6	0.263	209.00
MCM2	MCM2, BM28, CCNL1, CDCL1, KIAA0030: DNA replication licensing factor MCM2	0.263	1.29E3
MELK	MELK, KIAA0175: Maternal embryonic leucine zipper kinase	0.263	0.053
MCM4	MCM4, CDC21: DNA replication licensing factor MCM4	0.263	1.29E3
MCM7	MCM7, CDC47, MCM2: DNA replication licensing factor MCM7	0.263	209.00
PBK	PBK, TOPK: Lymphokine-activated killer T-cell-originated protein kinase	0.263	0.146
PCNA	PCNA: Proliferating cell nuclear antigen	0.242	1.62
PLK4	PLK4, SAK, STK18: Serine/threonine-protein kinase PLK4	0.207	0.207
E2F1	E2F1, RBBP3: Transcription factor E2F1	0.137	0.852
SNF1LK2	SNF1LK2, KIAA0781, QIK: Serine/threonine-protein kinase SNF1-like kinase 2	0.123	0.118
CDK4	CDK4: Cell division protein kinase 4	0.123	0.515
CHEK1	CHEK1, CHK1: Serine/threonine-protein kinase Chk1	0.123	0.515
MCM5	MCM5, CDC46: DNA replication licensing factor MCM5	0.123	209.00
BRCA1	BRCA1, RNF53: Breast cancer type 1 susceptibility protein	0.123	0.276
ORC1L	ORC1L, ORC1, PARC1: Origin recognition complex subunit 1	0.123	0.763
TFDP1	TFDP1, DP1: Transcription factor Dp-1	0.123	0.060
DDX3X	DDX3X, DBX, DDX3: ATP-dependent RNA helicase DDX3X	0.108	0.188
TPX2	TPX2, C20orf1, C20orf2, DIL2, HCA519: Targeting protein for Xklp2	0.082	0.053
DLG7	DLG7, KIAA0008: Disks large-associated protein DLG7	0.082	0.025
HMMR	HMMR: Hyaluronan-mediated motility receptor	0.082	0.027
ZWINT	N/A	0.082	0.027
BUB1B	BUB1B, BUBR1, MAD3L: Mitotic checkpoint serine/threonine-protein kinase BUB1 bet	0.081	0.053
HMGB2	HMGB2, HMG2: High mobility group protein B2	0.081	0.276
MYBL2	MYBL2, BMYB: Myb-related protein B	0.081	0.129
BUB1		0.081	0.053
CSNK2A1	CSNK2A1: CSNK2A1 protein	0.074	0.515
PRKCB1	N/A	0.071	0.025
RB1	RB1: Retinoblastoma-associated protein	0.067	0.515
GTF2B	GTF2B, TF2B, TFIIB: Transcription initiation factor IIB	0.060	0.372
GTF2F1	GTF2F1, RAP74: Transcription initiation factor IIF subunit alpha	0.060	0.461
CCNG1	CCNG1, CCNG, CYCG1: Cyclin-G1	0.060	0.123
H1F0	H1F0, H1FV: Histone H1.0	0.060	0.046
SKIIP	SNW1, SKIIP, SKIP: SNW domain-containing protein 1	0.060	0.129
CDK7	CDK7, MO15: Cell division protein kinase 7	0.060	0.118
OXSR1	OXSR1, KIAA1101, OSR1: Serine/threonine-protein kinase OSR1	0.060	0.241
STK11	STK11, LKB1, PJS: Serine/threonine-protein kinase 11	0.060	0.071
PHB2	PHB2, BAP, REA: Prohibitin-2	0.053	0.0446
RFC4	RFC4: Replication factor C subunit 4	0.051	0.207
TRIP13	TRIP13: Thyroid receptor-interacting protein 13	0.051	0.503
RFC3	RFC3: Replication factor C subunit 3	0.051	0.207
E2F2	E2F2: Transcription factor E2F2	0.050	0.0308
RFX5	RFX5: DNA-binding protein RFX5	0.050	0.137
MAPK6	MAPK6, ERK3, PRKM6: Mitogen-activated protein kinase 6	0.050	0.163
TOP2A	TOP2A, TOP2: DNA topoisomerase 2-alpha	0.049	0.307
MAP2K2	MAP2K2, MEK2, MKK2, PRKMK2: Dual specificity mitogen-activated protein kinase ki	0.047	0.090
DYRK3	N/A	0.044	0.043
HSF1	HSF1, HSTF1: Heat shock factor protein 1	0.044	0.225
GTF2E2	GTF2E2, TF2E2: Transcription initiation factor IIE subunit beta	0.044	0.074
NEK9	NEK9, KIAA1995, NEK8, NERCC: Serine/threonine-protein kinase Nek9	0.044	0.146
AKAP8L	AKAP8L, NAKAP95, HRIHFB2018: A-kinase anchor protein 8-like	0.044	0.038
CDC20	CDC20: Cell division cycle protein 20 homolog	0.044	0.276
CCT2	CCT2, 99D8.1, CCTB: T-complex protein 1 subunit beta	0.042	0.141
IRF4	IRF4, MUM1: Interferon regulatory factor 4	0.041	0.067
GTF2F2	N/A	0.041	0.251
STK16	N/A	0.041	0.080
MAP4K1	MAP4K1, HPK1: Mitogen-activated protein kinase kinase kinase kinase 1	0.041	0.163
PKMYT1	PKMYT1, MYT1: Membrane-associated tyrosine- and threonine-specific cdc2-inhibito	0.038	0.146
EIF2S1	EIF2S1, EIF2A: Eukaryotic translation initiation factor 2 subunit 1	0.038	1.62
BARD1	BARD1: BRCA1-associated RING domain protein 1	0.038	0.515
CBX3	CBX3: Chromobox protein homolog 3	0.038	0.276
RPA1	RPA1, REPA1, RPA70: Replication protein A 70 kDa DNA-binding subunit	0.038	0.263
VRK1	VRK1: Serine/threonine-protein kinase VRK1	0.038	0.515
YBX1	YBX1, NSEP1, YB1: Nuclease sensitive element-binding protein 1	0.038	0.763
SSRP1	SSRP1, FACT80: FACT complex subunit SSRP1	0.038	0.827
WEE1	WEE1: Wee1-like protein kinase	0.038	0.146
PRPF4B	PRPF4B, KIAA0536, PRP4, PRP4H, PRP4K: Serine/threonine-protein kinase PRP4 homol	0.036	0.146
PKN2	PKN2, PRK2, PRKCL2: Serine/threonine-protein kinase N2	0.036	0.087
RPS6KB1	N/A	0.036	0.118
CUL2	CUL2: Cullin-2	0.036	0.156
RPA2	RPA2, REPA2, RPA32, RPA34: Replication protein A 32 kDa subunit	0.036	0.372
CCNC	CCNC: Cyclin-C	0.036	0.060
CCND2	CCND2: G1/S-specific cyclin-D2	0.036	0.060
PIP5K3	PIP5K3, KIAA0981, PIKFYVE: FYVE finger-containing phosphoinositide kinase	0.036	0.461
AKAP8	AKAP8, AKAP95: A-kinase anchor protein 8	0.036	0.078
DVL2	DVL2: Segment polarity protein dishevelled homolog DVL-2	0.035	0.059
ROCK1	ROCK1: Rho-associated protein kinase 1	0.0308	0.0971
PRKAA1	N/A	0.0308	0.048
IRF5	IRF5: Interferon regulatory factor 5	0.0308	0.050
ZAK	MLTK, ZAK, HCCS4: Mitogen-activated protein kinase kinase kinase MLT	0.0308	0.048
NEK1	NEK1, KIAA1901: Serine/threonine-protein kinase Nek1	0.0308	0.060
ELF1	ELF1: ETS-related transcription factor Elf-1	0.0308	0.416
TLK1	TLK1, KIAA0137: Serine/threonine-protein kinase tousled-like 1	0.0308	0.132
PASK	PASK, KIAA0135: PAS domain-containing serine/threonine-protein kinase	0.0308	0.080
FOXJ2	FOXJ2, FHX: Forkhead box protein J2	0.0308	0.191
ADAR	ADAR, ADAR1, DSRAD, G1P1, IFI4: Double-stranded RNA-specific adenosine deaminase	0.0308	0.067
E2F3	E2F3, KIAA0075: Transcription factor E2F3	0.028	0.060
HSF2	HSF2, HSTF2: Heat shock factor protein 2	0.028	0.275
TCEB3	TCEB3, MSTP059: Transcription elongation factor B polypeptide 3	0.028	0.038
CDK6	CDK6: Cell division protein kinase 6	0.028	0.071
E2F5	E2F5: Transcription factor E2F5	0.028	0.036
GABPA	GABPA, E4TF1A: GA-binding protein alpha chain	0.028	0.044
STK3	STK3, MST2: Serine/threonine-protein kinase 3	0.028	0.071
RPS6KA1	RPS6KA1, RSK1: Ribosomal protein S6 kinase alpha-1	0.028	0.087
MAPK12	MAPK12, ERK6, SAPK3: Mitogen-activated protein kinase 12	0.028	0.054
CCND3	CCND3: G1/S-specific cyclin-D3	0.028	0.044
STK25	STK25, SOK1, YSK1: Serine/threonine-protein kinase 25	0.028	0.071
ADRBK1	ADRBK1, BARK, BARK1, GRK2: Beta-adrenergic receptor kinase 1	0.028	0.087
CCNH	CCNH: Cyclin-H	0.028	0.225
RPS6KB2	RPS6KB2, STK14B: Ribosomal protein S6 kinase beta-2	0.028	0.087
PSKH1	PSKH1: Serine/threonine-protein kinase H1	0.028	0.054
ZWILCH	Protein zwilch homolog.	0.027	0.027
TMPO	N/A	0.027	0.027
TACC3	TACC3, ERIC1: Transforming acidic coiled-coil-containing protein 3	0.027	0.025
GTSE1	GTSE1: G2 and S phase-expressed protein 1	0.027	0.025
CDCA8	CDCA8, PESCRG3: Borealin	0.027	0.027
GMNN	GMNN: Geminin	0.027	0.053
KIAA0101	PAF, KIAA0101, L5, NS5ATP9: PCNA-associated factor	0.027	0.027
C18orf56	N/A	0.027	0.082
SMC4L1	N/A	0.027	0.027
DBF4	DBF4, ASK: Protein DBF4 homolog	0.027	0.025
BIRC5	BIRC5, API4, IAP4: Baculoviral IAP repeat-containing protein 5	0.027	0.074
PKN1	PKN1, PKN, PRK1, PRKCL1: Serine/threonine-protein kinase N1	0.027	0.052
IRF7	IRF7: Interferon regulatory factor 7	0.027	0.027
HSFY1	HSFY1, HSF2L, HSFY: Heat shock transcription factor, Y-linked	0.027	0.027
MAPK9	MAPK9, JNK2, PRKM9: Mitogen-activated protein kinase 9	0.027	0.052
MINK1	MAP4K6, MINK1, MINK: Misshapen-like kinase 1	0.027	0.052
HTATIP	HTATIP, TIP60: Histone acetyltransferase HTATIP	0.027	0.165
HILS1	HILS1: Spermatid-specific linker histone H1-like protein	0.027	0.027
SPIC	SPIC: Transcription factor Spi-C	0.027	0.027
PAX3	PAX3, HUP2: Paired box protein Pax-3	0.027	0.027
MAPK15	N/A	0.027	0.052
FOXM1	FOXM1, FKHL16, HFH11, MPP2, WIN: Forkhead box protein M1	0.027	0.165
ELF2	ELF2, NERF: ETS-related transcription factor Elf-2	0.027	0.165
FBXO4	FBXO4, FBX4: F-box only protein 4	0.027	0.028
C20orf116	C20orf116: Uncharacterized protein C20orf116 precursor	0.027	0.027
FOXQ1	FOXQ1, HFH1: Forkhead box protein Q1	0.027	0.027
DHX15	DHX15, DBP1, DDX15: Putative pre-mRNA-splicing factor ATP-dependent RNA helicase	0.0255	0.293
BAZ1B	BAZ1B, WBSC10, WBSCR10, WBSCR9, WSTF: Bromodomain adjacent to zinc finger domain	0.0255	0.144
TRIM28	TRIM28, KAP1, RNF96, TIF1B: Transcription intermediary factor 1-beta	0.0255	0.307

0.572

Total Score

1.59E4