PIPs: Human Protein-Protein Interaction Prediction

Evidence of Interaction Between PCNA and RFC4

The interaction score between PCNA and RFC4 is 1.25E4.

This page provides the details about the evidence that was used to calculate the interaction Score between the two proteins

The Scores that are listed with each module are based on the interaction score where that module provides the only source of evidence. The final Score is the integration of all the sources of evidence.

Feature

Evidence

Score (?)

Gene Expression

Pearson's Correlation between PCNA and RFC4 = 0.89

The original expression data was collated from the NCBI GEO database. For further information about this dataset please refer to the NCBI GEO dataset GDS596.

0.027

Orthology

Organism	PCNA Orthologue		RFC4 Orthologue		Experiment
Organism	Accession	Inparalog Score	Accession	Inparalog Score	Experiment
bakers yeast	YBR088C	1	YJR068W	1	AffinityChromatography ReconstitutedComplex SyntheticRescue Get References From PubMed
worm	O02115	1	P53016	1
fly	P17917	1	Q9VX15	1

The Inparalog Score is calculated by InParanoid

The experiment type Other refers to techniques other than Y2H, MS, Immunoprecipitation, SurfacePlasmonResonance, XrayDiffraction, Xlinking, CompetitionBinding, Immunofluorescence, GelRetardationAssay, AffinityChromatography, InVitroBinding, InVitro, InVivo, FarWestern, pullDown, AffinityCaptureMS, EpistaticMiniArrayProfile, AffinityCaptureWestern, BiochemicalActivity, CoCrystalStructure, FRET, ReconstitutedComplex, CoLocalization, CoPurification or CoFractionation.

0.593

Domains

No domains were used in calculating likelihood of interaction

0.021

Post-translational Modifications

Protein: PCNA

PTM	Residue	Reference
Ubiquitination	164	PubMed, HPRD

Protein: RFC4

PTM	Residue	Reference

Localisation

Protein: PCNA

Localisation	Reference
Nucleus	HPRD
Nucleolus	HPRD
Cytoplasm	HPRD

Protein: RFC4

Localisation	Reference
Nucleoplasm	HPRD

Transitive Score

The common interactors between PCNA and RFC4 that are considered by the Transitive module are listed below.

The Scores listed are the values used by the transitive module to calculate the final interaction Score between PCNA andRFC4. The values listed are therefore not the final Score value for the listed interactions.

With a Score value ≥0.025 , PCNA has 851 interactors and RFC4 has 123 interactors of which there are 75 common interactors.

Common Interactor	Name of Common Interactor	Score for PCNA-Interactor (?)	Score for RFC4-Interactor (?)
RFC1	N/A	80.90	9.26
RFC5	RFC5: Replication factor C subunit 5	54.70	691.00
RFC3	RFC3: Replication factor C subunit 3	54.70	1.05E4
FEN1	FEN1, RAD2: Flap endonuclease 1	38.00	0.0739
IMPDH2	IMPDH2, IMPD2: Inosine-5'-monophosphate dehydrogenase 2	10.50	3.89
RFC2	RFC2: Replication factor C subunit 2	4.98	62.80
CHTF18	RUVBL, CHTF18: Protein CTF18 homolog.	3.39	0.354
MCM3	MCM3: DNA replication licensing factor MCM3	1.62	0.207
CDC6	CDC6, CDC18L: Cell division control protein 6 homolog	1.62	0.207
ATAD2	ATAD2, L16, PRO2000: ATPase family AAA domain-containing protein 2	1.07	0.123
MCM4	MCM4, CDC21: DNA replication licensing factor MCM4	0.827	0.207
MCM2	MCM2, BM28, CCNL1, CDCL1, KIAA0030: DNA replication licensing factor MCM2	0.827	0.207
CCNB1	Cyclin B1	0.822	0.051
MCM7	MCM7, CDC47, MCM2: DNA replication licensing factor MCM7	0.789	0.207
ORC1L	ORC1L, ORC1, PARC1: Origin recognition complex subunit 1	0.763	0.0968
BARD1	BARD1: BRCA1-associated RING domain protein 1	0.763	0.042
IMPDH1	IMPDH1: Uncharacterized protein IMPDH1	0.593	0.593
TRIP13	TRIP13: Thyroid receptor-interacting protein 13	0.304	0.123
PSMC2	PSMC2, MSS1: 26S protease regulatory subunit 7	0.304	0.074
PSMC1	PSMC1: 26S protease regulatory subunit 4	0.304	0.074
MDN1	MDN1, KIAA0301: Midasin	0.275	0.060
VCP	VCP: Transitional endoplasmic reticulum ATPase	0.245	0.044
CCNB2	CCNB2: G2/mitotic-specific cyclin-B2	0.242	0.051
CCNA2	CCNA2, CCN1, CCNA: Cyclin-A2	0.242	0.051
SRPR	SRPR: Uncharacterized protein SRPR	0.166	0.041
GMNN	GMNN: Geminin	0.160	0.027
PRKDC	PRKDC, HYRC, HYRC1: DNA-dependent protein kinase catalytic subunit	0.144	0.059
CSE1L	CSE1L, CAS, XPO2: Exportin-2	0.141	0.053
DHX15	DHX15, DBP1, DDX15: Putative pre-mRNA-splicing factor ATP-dependent RNA helicase	0.137	0.108
POLR2H	POLR2H: DNA-directed RNA polymerases I, II, and III subunit RPABC3	0.129	0.0968
YBX1	YBX1, NSEP1, YB1: Nuclease sensitive element-binding protein 1	0.129	0.123
EIF2S1	EIF2S1, EIF2A: Eukaryotic translation initiation factor 2 subunit 1	0.129	0.207
ABCE1	ABCE1, RLI, OK/SW-cl.40, RNASEL1, RNASELI, RNS4I: ATP-binding cassette sub-famil	0.123	0.263
TAF9	AK6, AD-004, CGI-137: Adenylate kinase isoenzyme 6	0.108	0.137
PSMC3	PSMC3, TBP1: 26S protease regulatory subunit 6A	0.0968	0.074
ATAD3A	ATAD3A: ATPase family AAA domain-containing protein 3A	0.0968	0.074
CCNF	CCNF: G2/mitotic-specific cyclin-F	0.090	0.027
C18orf56	N/A	0.082	0.027
KIAA0101	PAF, KIAA0101, L5, NS5ATP9: PCNA-associated factor	0.082	0.082
SMC4L1	N/A	0.082	0.082
ZWINT	N/A	0.082	0.027
KIAA0788	ASCC3L1, HELIC2, KIAA0788: U5 small nuclear ribonucleoprotein 200 kDa helicase	0.078	0.612
RP4-691N24.2	GINS1, KIAA0186, PSF1: DNA replication complex GINS protein PSF1	0.0739	0.0739
EIF5AP1	EIF5A: Eukaryotic translation initiation factor 5A-1	0.070	0.067
BRCA2	BRCA2, FACD, FANCD1: Breast cancer type 2 susceptibility protein	0.070	0.083
EIF2B5	EIF2B5, EIF2BE: Translation initiation factor eIF-2B subunit epsilon	0.063	0.059
YARS	YARS: Tyrosyl-tRNA synthetase, cytoplasmic	0.063	0.0968
ASCC3	ASCC3, HELIC1: Activating signal cointegrator 1 complex subunit 3	0.060	0.047
SRP54	SRP54: Signal recognition particle 54 kDa protein	0.059	0.074
PSMC6	PSMC6, SUG2: 26S protease regulatory subunit S10B	0.059	0.074
RUVBL1	RUVBL1, NMP238, TIP49, TIP49A: RuvB-like 1	0.059	0.123
TPX2	TPX2, C20orf1, C20orf2, DIL2, HCA519: Targeting protein for Xklp2	0.053	0.027
RFC1	RFC1, RFC140: Replication factor C subunit 1	0.051	0.0288
RUVBL2	RUVBL2, TIP48, CGI-46, TIP49B: RuvB-like 2	0.047	0.074
EXOSC2	EXOSC2, RRP4: Exosome complex exonuclease RRP4	0.046	0.060
RAD17	RAD17, R24L: Cell cycle checkpoint protein RAD17	0.046	4.78
MCM6	MCM6: DNA replication licensing factor MCM6	0.044	0.620
XAB1	XAB1, MBDIN, HUSSY-23: XPA-binding protein 1	0.0331	0.0331
FXR1	FXR1: Fragile X mental retardation syndrome-related protein 1	0.032	0.074
LAMR1	RPSA, LAMBR, LAMR1: 40S ribosomal protein SA	0.032	0.221
PEX1	PEX1: Peroxisome biogenesis factor 1	0.029	0.036
BCS1L	BCS1L, BCS1: Mitochondrial chaperone BCS1	0.029	0.044
VPS4A	VPS4A, VPS4: Vacuolar protein sorting-associating protein 4A	0.029	0.036
TMPO	N/A	0.027	0.027
C22orf18	N/A	0.027	0.027
CKAP2	CKAP2, LB1, TMAP: Cytoskeleton-associated protein 2	0.027	0.027
HMMR	HMMR: Hyaluronan-mediated motility receptor	0.027	0.027
RAD51AP1	N/A	0.027	0.027
MCM10	N/A	0.027	0.027
TPRKB	TPRKB, CGI-121, My019: TP53RK-binding protein	0.027	0.027
CDCA8	CDCA8, PESCRG3: Borealin	0.027	0.027
PRSS15	LONP1, PRSS15: Lon protease homolog, mitochondrial precursor	0.027	0.027
DNAH11	N/A	0.027	0.027
NOLC1	NOLC1, KIAA0035: Nucleolar phosphoprotein p130	0.025	0.027
DBF4	DBF4, ASK: Protein DBF4 homolog	0.025	0.027

0.572

Total Score

1.25E4