PIPs: Human Protein-Protein Interaction Prediction

Evidence of Interaction Between LIG1 and RFC1

The interaction score between LIG1 and RFC1 is 1.57.

This page provides the details about the evidence that was used to calculate the interaction Score between the two proteins

The Scores that are listed with each module are based on the interaction score where that module provides the only source of evidence. The final Score is the integration of all the sources of evidence.

Feature

Evidence

Score (?)

Gene Expression

Pearson's Correlation between LIG1 and RFC1 = 0.75

The original expression data was collated from the NCBI GEO database. For further information about this dataset please refer to the NCBI GEO dataset GDS596.

0.013

Orthology

Organism	LIG1 Orthologue		RFC1 Orthologue		Experiment
Organism	Accession	Inparalog Score	Accession	Inparalog Score	Experiment
bakers yeast	YDL164C	1	YOR217W	1
worm	Q27474	1	Q18841	1
fly	Q960P4	1	P35600	1

The Inparalog Score is calculated by InParanoid

The experiment type Other refers to techniques other than Y2H, MS, Immunoprecipitation, SurfacePlasmonResonance, XrayDiffraction, Xlinking, CompetitionBinding, Immunofluorescence, GelRetardationAssay, AffinityChromatography, InVitroBinding, InVitro, InVivo, FarWestern, pullDown, AffinityCaptureMS, EpistaticMiniArrayProfile, AffinityCaptureWestern, BiochemicalActivity, CoCrystalStructure, FRET, ReconstitutedComplex, CoLocalization, CoPurification or CoFractionation.

2.23E-3

Domains

No domains were used in calculating likelihood of interaction

1.48E-3

Post-translational Modifications

Protein: LIG1

PTM	Residue	Reference
Phosphorylation	66	PubMed, HPRD
Phosphorylation	76	PubMed, HPRD
Phosphorylation	141	PubMed, HPRD
Phosphorylation	195	PubMed, HPRD
Phosphorylation	911	PubMed, HPRD
Phosphoserine	66	HPRD
Phosphoserine	76	HPRD
Phosphoserine	141	HPRD
Phosphoserine	911	HPRD
Phosphothreonine	195	HPRD

Protein: RFC1

PTM	Residue	Reference

Localisation

Protein: LIG1

Localisation	Reference
Nucleus	HPRD
Nucleoplasm	HPRD

Protein: RFC1

Localisation	Reference
Nucleus	HPRD

Transitive Score

The common interactors between LIG1 and RFC1 that are considered by the Transitive module are listed below.

The Scores listed are the values used by the transitive module to calculate the final interaction Score between LIG1 andRFC1. The values listed are therefore not the final Score value for the listed interactions.

With a Score value ≥0.025 , LIG1 has 892 interactors and RFC1 has 74 interactors of which there are 26 common interactors.

Common Interactor	Name of Common Interactor	Score for LIG1-Interactor (?)	Score for RFC1-Interactor (?)
PCNA	PCNA: Proliferating cell nuclear antigen	73.00	80.90
ADPRT	PARP1, ADPRT, PPOL: Poly [ADP-ribose] polymerase 1	0.704	0.038
PRKDC	PRKDC, HYRC, HYRC1: DNA-dependent protein kinase catalytic subunit	0.307	0.137
MSH6	MSH6, GTBP: DNA mismatch repair protein MSH6	0.276	0.123
E2F1	E2F1, RBBP3: Transcription factor E2F1	0.144	0.083
MKI67	MKI67: Antigen KI-67	0.144	0.0255
CHD4	CHD4: Chromodomain-helicase-DNA-binding protein 4	0.144	0.0255
SMARCA4	SMARCA4, BRG1, SNF2B, SNF2L4: Probable global transcription activator SNF2L4	0.144	0.042
BRCA1	BRCA1, RNF53: Breast cancer type 1 susceptibility protein	0.129	0.074
SMC1L1	SMC1A, DXS423E, KIAA0178, SB1.8, SMC1, SMC1L1: Structural maintenance of chromos	0.129	0.060
CSPG6	SMC3, BAM, BMH, CSPG6, SMC3L1: Structural maintenance of chromosomes protein 3	0.129	0.074
H2AFX	H2AFX, H2AX: Histone H2A.x	0.129	15.90
POLA2	POLA2: DNA polymerase subunit alpha B	0.129	0.890
NBN	NBN, NBS, NBS1, P95: Nibrin	0.087	0.0255
RB1	RB1: Retinoblastoma-associated protein	0.087	0.0255
XRCC6	XRCC6, G22P1: ATP-dependent DNA helicase 2 subunit 1	0.087	0.067
ATR	ATR, FRP1: Serine/threonine-protein kinase ATR	0.078	0.074
TERF2	TERF2, TRBF2, TRF2: Telomeric repeat-binding factor 2	0.070	0.0255
LAMR1	RPSA, LAMBR, LAMR1: 40S ribosomal protein SA	0.066	0.221
IMPDH2	IMPDH2, IMPD2: Inosine-5'-monophosphate dehydrogenase 2	0.066	1.82
PIK3CD	PIK3CD: Phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta i	0.0425	0.041
ATM	ATM: Serine-protein kinase ATM	0.038	0.028
FRAP1	FRAP1, FRAP, FRAP2: FKBP12-rapamycin complex-associated protein	0.032	0.0308
POU2F1	POU2F1, OCT1, OTF1: POU domain, class 2, transcription factor 1	0.032	0.0308
HIST2H2AC	HIST2H2AC, H2AFQ: Histone H2A type 2-C	0.025	1.27
HIST3H2A	HIST3H2A: Histone H2A type 3	0.025	0.071

0.572

Total Score

1.57