PIPs: Human Protein-Protein Interaction Prediction

Evidence of Interaction Between MAPK1 and DUSP14

The interaction score between MAPK1 and DUSP14 is 46.80.

This page provides the details about the evidence that was used to calculate the interaction Score between the two proteins

The Scores that are listed with each module are based on the interaction score where that module provides the only source of evidence. The final Score is the integration of all the sources of evidence.

Feature

Evidence

Score (?)

Gene Expression

Pearson's Correlation between MAPK1 and DUSP14 = 0.07

The original expression data was collated from the NCBI GEO database. For further information about this dataset please refer to the NCBI GEO dataset GDS596.

1.85E-3

Orthology

Organism	MAPK1 Orthologue		DUSP14 Orthologue		Experiment
Organism	Accession	Inparalog Score	Accession	Inparalog Score	Experiment
bakers yeast	YBL016W	1	YBR276C	1
bakers yeast	YBL016W	1	YIL113W	1
bakers yeast	YBL016W	1	YNL053W	1	Immunoprecipitation CoLocalization Get References From PubMed
bakers yeast	YGR040W	0.096	YBR276C	1
bakers yeast	YGR040W	0.096	YIL113W	1
bakers yeast	YGR040W	0.096	YNL053W	1
worm	P39745	1	Q9XVE7	1
fly	P40417	1	Q9VAB0	1

The Inparalog Score is calculated by InParanoid

The experiment type Other refers to techniques other than Y2H, MS, Immunoprecipitation, SurfacePlasmonResonance, XrayDiffraction, Xlinking, CompetitionBinding, Immunofluorescence, GelRetardationAssay, AffinityChromatography, InVitroBinding, InVitro, InVivo, FarWestern, pullDown, AffinityCaptureMS, EpistaticMiniArrayProfile, AffinityCaptureWestern, BiochemicalActivity, CoCrystalStructure, FRET, ReconstitutedComplex, CoLocalization, CoPurification or CoFractionation.

0.593

Domains

Domains present in MAPK1 and DUSP14:

Domains in MAPK1

Domains in DUSP14

IPR000719	Protein kinase
IPR002290	Serine/threonine protein kinase
IPR011009	Protein kinase-like
IPR008271	Serine/threonine protein kinase, active site
IPR003527	MAP kinase
IPR008349	ERK1/2 MAP kinase

IPR000387	Tyrosine specific protein phosphatase and dual specificity protein phosphatase
IPR000340	Dual specificity protein phosphatase

The Chi square scores for co-occurrence of domains that are present in MAPK1 and DUSP14 are listed below.

Domain 1		Domain 2		Chi Square
IPR003527	MAP kinase	IPR000340	Dual specificity protein phosphatase	575.549
IPR008349	ERK1/2 MAP kinase	IPR000340	Dual specificity protein phosphatase	317.529
IPR003527	MAP kinase	IPR000387	Tyrosine specific protein phosphatase and dual specificity protein phosphatase	128.642
IPR002290	Serine/threonine protein kinase	IPR000340	Dual specificity protein phosphatase	108.157
IPR008349	ERK1/2 MAP kinase	IPR000387	Tyrosine specific protein phosphatase and dual specificity protein phosphatase	89.8437
IPR008271	Serine/threonine protein kinase, active site	IPR000340	Dual specificity protein phosphatase	76.5901
IPR000719	Protein kinase	IPR000340	Dual specificity protein phosphatase	30.0934
IPR011009	Protein kinase-like	IPR000340	Dual specificity protein phosphatase	15.3476
IPR000719	Protein kinase	IPR000387	Tyrosine specific protein phosphatase and dual specificity protein phosphatase	14.0817
IPR002290	Serine/threonine protein kinase	IPR000387	Tyrosine specific protein phosphatase and dual specificity protein phosphatase	10.8808
IPR011009	Protein kinase-like	IPR000387	Tyrosine specific protein phosphatase and dual specificity protein phosphatase	6.38835
IPR008271	Serine/threonine protein kinase, active site	IPR000387	Tyrosine specific protein phosphatase and dual specificity protein phosphatase	2.56073

The background colour of the table refers to:

Blue Domain is only present in MAPK1
Yellow Domain is only present in DUSP14
Green Domain is present in both MAPK1 and DUSP14

0.265

Post-translational Modifications

Protein: MAPK1

PTM	Residue	Reference
Acetylation	2	PubMed, HPRD
Dephosphorylation	187	PubMed, HPRD
N-acetylalanine	1	HPRD
Phosphorylation	29	PubMed, HPRD
Phosphorylation	41	PubMed, HPRD
Phosphorylation	181	PubMed, HPRD
Phosphorylation	185	PubMed, HPRD
Phosphorylation	187	PubMed, HPRD
Phosphorylation	202	PubMed, HPRD
Phosphothreonine	184	PubMed, HPRD
Phosphotyrosine	186	HPRD

Protein: DUSP14

PTM	Residue	Reference

Localisation

Protein: MAPK1

Localisation	Reference
Nucleus	HPRD
Cytoplasm	HPRD
Cytoskeleton	HPRD

Protein: DUSP14

Localisation	Reference
Nucleus	HPRD
Cytoplasm	HPRD

Transitive Score

No Transitive data was used in the prediction of this interaction.

N/A

Total Score

46.80