PIPs: Human Protein-Protein Interaction Prediction

Evidence of Interaction Between RFC4 and CCNB1

The interaction score between RFC4 and CCNB1 is 7.07.

This page provides the details about the evidence that was used to calculate the interaction Score between the two proteins

The Scores that are listed with each module are based on the interaction score where that module provides the only source of evidence. The final Score is the integration of all the sources of evidence.

Feature

Evidence

Score (?)

Gene Expression

Pearson's Correlation between RFC4 and CCNB1 = 0.84

The original expression data was collated from the NCBI GEO database. For further information about this dataset please refer to the NCBI GEO dataset GDS596.

0.027

Orthology

Organism	RFC4 Orthologue		CCNB1 Orthologue		Experiment
Organism	Accession	Inparalog Score	Accession	Inparalog Score	Experiment
bakers yeast	YJR068W	1	YPR119W	0.321
worm	P53016	1	Q10653	1
worm	P53016	1	O45926	0.463
worm	P53016	1	Q9BLB5	0.463
fly	Q9VX15	1	P20439	1

The Inparalog Score is calculated by InParanoid

The experiment type Other refers to techniques other than Y2H, MS, Immunoprecipitation, SurfacePlasmonResonance, XrayDiffraction, Xlinking, CompetitionBinding, Immunofluorescence, GelRetardationAssay, AffinityChromatography, InVitroBinding, InVitro, InVivo, FarWestern, pullDown, AffinityCaptureMS, EpistaticMiniArrayProfile, AffinityCaptureWestern, BiochemicalActivity, CoCrystalStructure, FRET, ReconstitutedComplex, CoLocalization, CoPurification or CoFractionation.

2.23E-3

Domains

Domains present in RFC4 and CCNB1:

Domains in RFC4

Domains in CCNB1

IPR003593	AAA ATPase
IPR003959	AAA ATPase, central region
IPR008921	DNA polymerase III clamp loader subunit, C-terminal
IPR000862	RFC
IPR013748	Replication factor C

IPR011028	Cyclin-like
IPR006670	Cyclin
IPR006671	Cyclin, N-terminal
IPR004367	Cyclin, C-terminal
IPR013763	Cyclin-related

The Chi square scores for co-occurrence of domains that are present in RFC4 and CCNB1 are listed below.

Domain 1		Domain 2		Chi Square
IPR003593	AAA ATPase	IPR006670	Cyclin	0.931553
IPR003593	AAA ATPase	IPR011028	Cyclin-like	0.663281
IPR003593	AAA ATPase	IPR013763	Cyclin-related	0.604607

The background colour of the table refers to:

Blue Domain is only present in RFC4
Yellow Domain is only present in CCNB1
Green Domain is present in both RFC4 and CCNB1

5.15E-3

Post-translational Modifications

Protein: RFC4

PTM	Residue	Reference

Protein: CCNB1

PTM	Residue	Reference
Phosphorylation	126	PubMed, HPRD
Phosphorylation	128	PubMed, HPRD
Phosphorylation	133	PubMed, HPRD
Phosphorylation	147	PubMed, HPRD

Localisation

Protein: RFC4

Localisation	Reference
Nucleoplasm	HPRD

Protein: CCNB1

Localisation	Reference
Nucleus	HPRD
Plasma membrane	HPRD
Nucleolus	HPRD
Centrosome	HPRD
Cytoplasm	HPRD
Cytoskeleton	HPRD

Transitive Score

The common interactors between RFC4 and CCNB1 that are considered by the Transitive module are listed below.

The Scores listed are the values used by the transitive module to calculate the final interaction Score between RFC4 andCCNB1. The values listed are therefore not the final Score value for the listed interactions.

With a Score value ≥0.025 , RFC4 has 123 interactors and CCNB1 has 1142 interactors of which there are 57 common interactors.

Common Interactor	Name of Common Interactor	Score for RFC4-Interactor (?)	Score for CCNB1-Interactor (?)
RFC3	RFC3: Replication factor C subunit 3	1.05E4	0.051
PCNA	PCNA: Proliferating cell nuclear antigen	54.70	0.822
IMPDH2	IMPDH2, IMPD2: Inosine-5'-monophosphate dehydrogenase 2	3.89	0.040
KPNA6	N/A	2.37	0.0732
KPNA5	KPNA5: Importin subunit alpha-6	1.18	0.0294
KPNA1	KPNA1, RCH2: Importin subunit alpha-1	0.647	0.0392
MCM6	MCM6: DNA replication licensing factor MCM6	0.620	0.123
KIAA0788	ASCC3L1, HELIC2, KIAA0788: U5 small nuclear ribonucleoprotein 200 kDa helicase	0.612	0.063
ABCE1	ABCE1, RLI, OK/SW-cl.40, RNASEL1, RNASELI, RNS4I: ATP-binding cassette sub-famil	0.263	0.038
LAMR1	RPSA, LAMBR, LAMR1: 40S ribosomal protein SA	0.221	0.032
EIF2S1	EIF2S1, EIF2A: Eukaryotic translation initiation factor 2 subunit 1	0.207	0.385
MCM2	MCM2, BM28, CCNL1, CDCL1, KIAA0030: DNA replication licensing factor MCM2	0.207	1.62
MCM3	MCM3: DNA replication licensing factor MCM3	0.207	40.60
MCM4	MCM4, CDC21: DNA replication licensing factor MCM4	0.207	1.62
CDC6	CDC6, CDC18L: Cell division control protein 6 homolog	0.207	86.60
MCM5	MCM5, CDC46: DNA replication licensing factor MCM5	0.207	0.123
MCM7	MCM7, CDC47, MCM2: DNA replication licensing factor MCM7	0.207	0.263
YBX1	YBX1, NSEP1, YB1: Nuclease sensitive element-binding protein 1	0.123	0.385
ATAD2	ATAD2, L16, PRO2000: ATPase family AAA domain-containing protein 2	0.123	12.00
TRIP13	TRIP13: Thyroid receptor-interacting protein 13	0.123	0.484
DHX15	DHX15, DBP1, DDX15: Putative pre-mRNA-splicing factor ATP-dependent RNA helicase	0.108	0.0255
RPA1	RPA1, REPA1, RPA70: Replication protein A 70 kDa DNA-binding subunit	0.0968	0.038
POLR2H	POLR2H: DNA-directed RNA polymerases I, II, and III subunit RPABC3	0.0968	0.045
YARS	YARS: Tyrosyl-tRNA synthetase, cytoplasmic	0.0968	0.233
ORC1L	ORC1L, ORC1, PARC1: Origin recognition complex subunit 1	0.0968	0.763
BRCA2	BRCA2, FACD, FANCD1: Breast cancer type 2 susceptibility protein	0.083	0.210
SMC4L1	N/A	0.082	0.027
KIAA0101	PAF, KIAA0101, L5, NS5ATP9: PCNA-associated factor	0.082	0.027
PLK4	PLK4, SAK, STK18: Serine/threonine-protein kinase PLK4	0.081	0.207
PSMC2	PSMC2, MSS1: 26S protease regulatory subunit 7	0.074	7.30
FXR1	FXR1: Fragile X mental retardation syndrome-related protein 1	0.074	0.111
PSMC1	PSMC1: 26S protease regulatory subunit 4	0.074	0.137
FEN1	FEN1, RAD2: Flap endonuclease 1	0.0739	0.0479
CKS2	CKS2: Cyclin-dependent kinases regulatory subunit 2	0.0739	1.31
EIF5AP1	EIF5A: Eukaryotic translation initiation factor 5A-1	0.067	0.260
EXOSC2	EXOSC2, RRP4: Exosome complex exonuclease RRP4	0.060	0.139
MDN1	MDN1, KIAA0301: Midasin	0.060	0.114
PRKDC	PRKDC, HYRC, HYRC1: DNA-dependent protein kinase catalytic subunit	0.059	0.144
ORC2L	ORC2L, ORC2: Origin recognition complex subunit 2	0.059	0.221
EIF2B5	EIF2B5, EIF2BE: Translation initiation factor eIF-2B subunit epsilon	0.059	0.139
CSE1L	CSE1L, CAS, XPO2: Exportin-2	0.053	0.033
CCNB2	CCNB2: G2/mitotic-specific cyclin-B2	0.051	0.789
CCNA2	CCNA2, CCN1, CCNA: Cyclin-A2	0.051	0.789
VCP	VCP: Transitional endoplasmic reticulum ATPase	0.044	0.111
BARD1	BARD1: BRCA1-associated RING domain protein 1	0.042	0.233
SRPR	SRPR: Uncharacterized protein SRPR	0.041	0.057
TPX2	TPX2, C20orf1, C20orf2, DIL2, HCA519: Targeting protein for Xklp2	0.027	0.053
CKAP2	CKAP2, LB1, TMAP: Cytoskeleton-associated protein 2	0.027	0.027
HMMR	HMMR: Hyaluronan-mediated motility receptor	0.027	0.082
TMPO	N/A	0.027	0.027
ZWINT	N/A	0.027	0.027
GMNN	GMNN: Geminin	0.027	0.053
DBF4	DBF4, ASK: Protein DBF4 homolog	0.027	0.025
C18orf56	N/A	0.027	0.027
CDCA8	CDCA8, PESCRG3: Borealin	0.027	0.027
CDCA3	CDCA3, C8, GRCC8, TOME1: Cell division cycle-associated protein 3	0.027	0.027
CCNF	CCNF: G2/mitotic-specific cyclin-F	0.027	0.293

0.347

Total Score

7.07