PIPs: Human Protein-Protein Interaction Prediction

Evidence of Interaction Between RFC4 and PCNA

The interaction score between RFC4 and PCNA is 1.25E4.

This page provides the details about the evidence that was used to calculate the interaction Score between the two proteins

The Scores that are listed with each module are based on the interaction score where that module provides the only source of evidence. The final Score is the integration of all the sources of evidence.

Feature

Evidence

Score (?)

Gene Expression

Pearson's Correlation between RFC4 and PCNA = 0.89

The original expression data was collated from the NCBI GEO database. For further information about this dataset please refer to the NCBI GEO dataset GDS596.

0.027

Orthology

Organism	RFC4 Orthologue		PCNA Orthologue		Experiment
Organism	Accession	Inparalog Score	Accession	Inparalog Score	Experiment
bakers yeast	YJR068W	1	YBR088C	1	AffinityChromatography ReconstitutedComplex SyntheticRescue Get References From PubMed
worm	P53016	1	O02115	1
fly	Q9VX15	1	P17917	1

The Inparalog Score is calculated by InParanoid

The experiment type Other refers to techniques other than Y2H, MS, Immunoprecipitation, SurfacePlasmonResonance, XrayDiffraction, Xlinking, CompetitionBinding, Immunofluorescence, GelRetardationAssay, AffinityChromatography, InVitroBinding, InVitro, InVivo, FarWestern, pullDown, AffinityCaptureMS, EpistaticMiniArrayProfile, AffinityCaptureWestern, BiochemicalActivity, CoCrystalStructure, FRET, ReconstitutedComplex, CoLocalization, CoPurification or CoFractionation.

0.593

Domains

No domains were used in calculating likelihood of interaction

0.021

Post-translational Modifications

Protein: RFC4

PTM	Residue	Reference

Protein: PCNA

PTM	Residue	Reference
Ubiquitination	164	PubMed, HPRD

Localisation

Protein: RFC4

Localisation	Reference
Nucleoplasm	HPRD

Protein: PCNA

Localisation	Reference
Nucleus	HPRD
Nucleolus	HPRD
Cytoplasm	HPRD

Transitive Score

The common interactors between RFC4 and PCNA that are considered by the Transitive module are listed below.

The Scores listed are the values used by the transitive module to calculate the final interaction Score between RFC4 andPCNA. The values listed are therefore not the final Score value for the listed interactions.

With a Score value ≥0.025 , RFC4 has 123 interactors and PCNA has 851 interactors of which there are 75 common interactors.

Common Interactor	Name of Common Interactor	Score for RFC4-Interactor (?)	Score for PCNA-Interactor (?)
RFC3	RFC3: Replication factor C subunit 3	1.05E4	54.70
RFC5	RFC5: Replication factor C subunit 5	691.00	54.70
RFC2	RFC2: Replication factor C subunit 2	62.80	4.98
RFC1	N/A	9.26	80.90
RAD17	RAD17, R24L: Cell cycle checkpoint protein RAD17	4.78	0.046
IMPDH2	IMPDH2, IMPD2: Inosine-5'-monophosphate dehydrogenase 2	3.89	10.50
MCM6	MCM6: DNA replication licensing factor MCM6	0.620	0.044
KIAA0788	ASCC3L1, HELIC2, KIAA0788: U5 small nuclear ribonucleoprotein 200 kDa helicase	0.612	0.078
IMPDH1	IMPDH1: Uncharacterized protein IMPDH1	0.593	0.593
CHTF18	RUVBL, CHTF18: Protein CTF18 homolog.	0.354	3.39
ABCE1	ABCE1, RLI, OK/SW-cl.40, RNASEL1, RNASELI, RNS4I: ATP-binding cassette sub-famil	0.263	0.123
LAMR1	RPSA, LAMBR, LAMR1: 40S ribosomal protein SA	0.221	0.032
MCM4	MCM4, CDC21: DNA replication licensing factor MCM4	0.207	0.827
CDC6	CDC6, CDC18L: Cell division control protein 6 homolog	0.207	1.62
MCM7	MCM7, CDC47, MCM2: DNA replication licensing factor MCM7	0.207	0.789
EIF2S1	EIF2S1, EIF2A: Eukaryotic translation initiation factor 2 subunit 1	0.207	0.129
MCM2	MCM2, BM28, CCNL1, CDCL1, KIAA0030: DNA replication licensing factor MCM2	0.207	0.827
MCM3	MCM3: DNA replication licensing factor MCM3	0.207	1.62
TAF9	AK6, AD-004, CGI-137: Adenylate kinase isoenzyme 6	0.137	0.108
RUVBL1	RUVBL1, NMP238, TIP49, TIP49A: RuvB-like 1	0.123	0.059
TRIP13	TRIP13: Thyroid receptor-interacting protein 13	0.123	0.304
YBX1	YBX1, NSEP1, YB1: Nuclease sensitive element-binding protein 1	0.123	0.129
ATAD2	ATAD2, L16, PRO2000: ATPase family AAA domain-containing protein 2	0.123	1.07
DHX15	DHX15, DBP1, DDX15: Putative pre-mRNA-splicing factor ATP-dependent RNA helicase	0.108	0.137
POLR2H	POLR2H: DNA-directed RNA polymerases I, II, and III subunit RPABC3	0.0968	0.129
YARS	YARS: Tyrosyl-tRNA synthetase, cytoplasmic	0.0968	0.063
ORC1L	ORC1L, ORC1, PARC1: Origin recognition complex subunit 1	0.0968	0.763
BRCA2	BRCA2, FACD, FANCD1: Breast cancer type 2 susceptibility protein	0.083	0.070
KIAA0101	PAF, KIAA0101, L5, NS5ATP9: PCNA-associated factor	0.082	0.082
SMC4L1	N/A	0.082	0.082
PSMC1	PSMC1: 26S protease regulatory subunit 4	0.074	0.304
PSMC6	PSMC6, SUG2: 26S protease regulatory subunit S10B	0.074	0.059
ATAD3A	ATAD3A: ATPase family AAA domain-containing protein 3A	0.074	0.0968
PSMC3	PSMC3, TBP1: 26S protease regulatory subunit 6A	0.074	0.0968
PSMC2	PSMC2, MSS1: 26S protease regulatory subunit 7	0.074	0.304
RUVBL2	RUVBL2, TIP48, CGI-46, TIP49B: RuvB-like 2	0.074	0.047
FXR1	FXR1: Fragile X mental retardation syndrome-related protein 1	0.074	0.032
SRP54	SRP54: Signal recognition particle 54 kDa protein	0.074	0.059
FEN1	FEN1, RAD2: Flap endonuclease 1	0.0739	38.00
RP4-691N24.2	GINS1, KIAA0186, PSF1: DNA replication complex GINS protein PSF1	0.0739	0.0739
EIF5AP1	EIF5A: Eukaryotic translation initiation factor 5A-1	0.067	0.070
MDN1	MDN1, KIAA0301: Midasin	0.060	0.275
EXOSC2	EXOSC2, RRP4: Exosome complex exonuclease RRP4	0.060	0.046
PRKDC	PRKDC, HYRC, HYRC1: DNA-dependent protein kinase catalytic subunit	0.059	0.144
EIF2B5	EIF2B5, EIF2BE: Translation initiation factor eIF-2B subunit epsilon	0.059	0.063
CSE1L	CSE1L, CAS, XPO2: Exportin-2	0.053	0.141
CCNB2	CCNB2: G2/mitotic-specific cyclin-B2	0.051	0.242
CCNA2	CCNA2, CCN1, CCNA: Cyclin-A2	0.051	0.242
CCNB1	Cyclin B1	0.051	0.822
ASCC3	ASCC3, HELIC1: Activating signal cointegrator 1 complex subunit 3	0.047	0.060
BCS1L	BCS1L, BCS1: Mitochondrial chaperone BCS1	0.044	0.029
VCP	VCP: Transitional endoplasmic reticulum ATPase	0.044	0.245
BARD1	BARD1: BRCA1-associated RING domain protein 1	0.042	0.763
SRPR	SRPR: Uncharacterized protein SRPR	0.041	0.166
PEX1	PEX1: Peroxisome biogenesis factor 1	0.036	0.029
VPS4A	VPS4A, VPS4: Vacuolar protein sorting-associating protein 4A	0.036	0.029
XAB1	XAB1, MBDIN, HUSSY-23: XPA-binding protein 1	0.0331	0.0331
RFC1	RFC1, RFC140: Replication factor C subunit 1	0.0288	0.051
DBF4	DBF4, ASK: Protein DBF4 homolog	0.027	0.025
C18orf56	N/A	0.027	0.082
CDCA8	CDCA8, PESCRG3: Borealin	0.027	0.027
NOLC1	NOLC1, KIAA0035: Nucleolar phosphoprotein p130	0.027	0.025
CKAP2	CKAP2, LB1, TMAP: Cytoskeleton-associated protein 2	0.027	0.027
TPX2	TPX2, C20orf1, C20orf2, DIL2, HCA519: Targeting protein for Xklp2	0.027	0.053
HMMR	HMMR: Hyaluronan-mediated motility receptor	0.027	0.027
TPRKB	TPRKB, CGI-121, My019: TP53RK-binding protein	0.027	0.027
MCM10	N/A	0.027	0.027
TMPO	N/A	0.027	0.027
C22orf18	N/A	0.027	0.027
ZWINT	N/A	0.027	0.082
GMNN	GMNN: Geminin	0.027	0.160
RAD51AP1	N/A	0.027	0.027
PRSS15	LONP1, PRSS15: Lon protease homolog, mitochondrial precursor	0.027	0.027
DNAH11	N/A	0.027	0.027
CCNF	CCNF: G2/mitotic-specific cyclin-F	0.027	0.090

0.572

Total Score

1.25E4