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Evidence of Interaction Between RFC4 and CDK2

The interaction score between RFC4 and CDK2 is 1.75.

This page provides the details about the evidence that was used to calculate the interaction Score between the two proteins

The Scores that are listed with each module are based on the interaction score where that module provides the only source of evidence. The final Score is the integration of all the sources of evidence.

FeatureEvidenceScore (?)
Gene Expression

Pearson's Correlation between RFC4 and CDK2 = 0.82

The original expression data was collated from the NCBI GEO database. For further information about this dataset please refer to the NCBI GEO dataset GDS596.

 0.027
Orthology
OrganismRFC4 OrthologueCDK2 OrthologueExperiment
AccessionInparalog ScoreAccessionInparalog Score
bakers yeast YJR068W 1 YBR160W 0.352
fly Q9VX15 1 P23573 1

The Inparalog Score is calculated by InParanoid

The experiment type Other refers to techniques other than Y2H, MS, Immunoprecipitation, SurfacePlasmonResonance, XrayDiffraction, Xlinking, CompetitionBinding, Immunofluorescence, GelRetardationAssay, AffinityChromatography, InVitroBinding, InVitro, InVivo, FarWestern, pullDown, AffinityCaptureMS, EpistaticMiniArrayProfile, AffinityCaptureWestern, BiochemicalActivity, CoCrystalStructure, FRET, ReconstitutedComplex, CoLocalization, CoPurification or CoFractionation.

 2.23E-3
Domains Domains present in RFC4 and CDK2:
Domains in RFC4Domains in CDK2
IPR003593 AAA ATPase
IPR003959 AAA ATPase, central region
IPR008921 DNA polymerase III clamp loader subunit, C-terminal
IPR000862 RFC
IPR013748 Replication factor C
IPR000719 Protein kinase
IPR002290 Serine/threonine protein kinase
IPR011009 Protein kinase-like
IPR008271 Serine/threonine protein kinase, active site

The Chi square scores for co-occurrence of domains that are present in RFC4 and CDK2 are listed below.

Domain 1Domain 2Chi Square
IPR003593 AAA ATPase IPR000719 Protein kinase 12.6046
IPR003593 AAA ATPase IPR011009 Protein kinase-like 6.88557
IPR003959 AAA ATPase, central region IPR000719 Protein kinase 6.2595
IPR003593 AAA ATPase IPR002290 Serine/threonine protein kinase 3.98716
IPR003959 AAA ATPase, central region IPR011009 Protein kinase-like 3.5153
IPR003593 AAA ATPase IPR008271 Serine/threonine protein kinase, active site 3.41163
IPR003959 AAA ATPase, central region IPR008271 Serine/threonine protein kinase, active site 1.79861

The background colour of the table refers to:

  • Blue Domain is only present in RFC4
  • Yellow Domain is only present in CDK2
  • Green Domain is present in both RFC4 and CDK2

 1.48E-3
Post-translational Modifications

Protein: RFC4

PTMResidueReference

Protein: CDK2

PTMResidueReference
Dephosphorylation 14 PubMed, HPRD
Dephosphorylation 15 PubMed, HPRD
Dephosphorylation 160 PubMed, HPRD
Phosphorylation 14 PubMed, HPRD
Phosphorylation 15 PubMed, HPRD
Phosphorylation 160 PubMed, HPRD
Phosphothreonine 14 HPRD
Phosphothreonine 160 PubMed, HPRD
Phosphotyrosine 15 PubMed, HPRD
Localisation

Protein: RFC4

LocalisationReference
Nucleoplasm HPRD

Protein: CDK2

LocalisationReference
Nucleus HPRD
Nucleolus HPRD
Cytoplasm HPRD
Transitive Score

The common interactors between RFC4 and CDK2 that are considered by the Transitive module are listed below.

The Scores listed are the values used by the transitive module to calculate the final interaction Score between RFC4 andCDK2. The values listed are therefore not the final Score value for the listed interactions.

With a Score value ≥0.025 , RFC4 has 123 interactors and CDK2 has 1600 interactors of which there are 44 common interactors.

Common InteractorName of Common InteractorScore for RFC4-Interactor (?)Score for CDK2-Interactor (?)
PCNA PCNA: Proliferating cell nuclear antigen 54.70 0.276
IMPDH2 IMPDH2, IMPD2: Inosine-5'-monophosphate dehydrogenase 2 3.89 0.233
KPNA5 KPNA5: Importin subunit alpha-6 1.18 1.15
KIAA0788 ASCC3L1, HELIC2, KIAA0788: U5 small nuclear ribonucleoprotein 200 kDa helicase 0.612 0.078
CHTF18 RUVBL, CHTF18: Protein CTF18 homolog. 0.354 0.025
GART GART, PGFT, PRGS: Trifunctional purine biosynthetic protein adenosine-3 [Include 0.354 0.075
LAMR1 RPSA, LAMBR, LAMR1: 40S ribosomal protein SA 0.221 0.063
MCM4 MCM4, CDC21: DNA replication licensing factor MCM4 0.207 0.276
CDC6 CDC6, CDC18L: Cell division control protein 6 homolog 0.207 6.86
EIF2S1 EIF2S1, EIF2A: Eukaryotic translation initiation factor 2 subunit 1 0.207 0.129
MCM2 MCM2, BM28, CCNL1, CDCL1, KIAA0030: DNA replication licensing factor MCM2 0.207 0.515
MCM3 MCM3: DNA replication licensing factor MCM3 0.207 27.40
TRIP13 TRIP13: Thyroid receptor-interacting protein 13 0.123 0.078
YBX1 YBX1, NSEP1, YB1: Nuclease sensitive element-binding protein 1 0.123 0.078
ATAD2 ATAD2, L16, PRO2000: ATPase family AAA domain-containing protein 2 0.123 4.15
YARS YARS: Tyrosyl-tRNA synthetase, cytoplasmic 0.0968 0.078
ORC1L ORC1L, ORC1, PARC1: Origin recognition complex subunit 1 0.0968 0.129
BRCA2 BRCA2, FACD, FANCD1: Breast cancer type 2 susceptibility protein 0.083 0.260
KIAA0101 PAF, KIAA0101, L5, NS5ATP9: PCNA-associated factor 0.082 0.027
PLK4 PLK4, SAK, STK18: Serine/threonine-protein kinase PLK4 0.081 0.038
PSMC1 PSMC1: 26S protease regulatory subunit 4 0.074 0.063
PSMC2 PSMC2, MSS1: 26S protease regulatory subunit 7 0.074 4.15
FXR1 FXR1: Fragile X mental retardation syndrome-related protein 1 0.074 0.063
CKS2 CKS2: Cyclin-dependent kinases regulatory subunit 2 0.0739 15.50
FEN1 FEN1, RAD2: Flap endonuclease 1 0.0739 0.0479
EIF5AP1 EIF5A: Eukaryotic translation initiation factor 5A-1 0.067 0.070
MDN1 MDN1, KIAA0301: Midasin 0.060 0.046
EXOSC2 EXOSC2, RRP4: Exosome complex exonuclease RRP4 0.060 0.046
PRKDC PRKDC, HYRC, HYRC1: DNA-dependent protein kinase catalytic subunit 0.059 0.430
EIF2B5 EIF2B5, EIF2BE: Translation initiation factor eIF-2B subunit epsilon 0.059 0.188
ORC2L ORC2L, ORC2: Origin recognition complex subunit 2 0.059 0.221
CSE1L CSE1L, CAS, XPO2: Exportin-2 0.053 0.129
CCNB2 CCNB2: G2/mitotic-specific cyclin-B2 0.051 6.55
CCNA2 CCNA2, CCN1, CCNA: Cyclin-A2 0.051 0.263
CCNB1 Cyclin B1 0.051 21.70
VCP VCP: Transitional endoplasmic reticulum ATPase 0.044 0.063
BARD1 BARD1: BRCA1-associated RING domain protein 1 0.042 0.144
SRPR SRPR: Uncharacterized protein SRPR 0.041 0.0425
C18orf56 N/A 0.027 0.027
NOLC1 NOLC1, KIAA0035: Nucleolar phosphoprotein p130 0.027 0.025
TPX2 TPX2, C20orf1, C20orf2, DIL2, HCA519: Targeting protein for Xklp2 0.027 0.053
ZWINT N/A 0.027 0.027
GMNN GMNN: Geminin 0.027 0.025
CCNF CCNF: G2/mitotic-specific cyclin-F 0.027 0.083
0.299
Total Score 1.75