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Publications: 1994 |
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Russell, R. B. and Barton, G. J. (1994), Proteins with similar folds but very different sequences can show few common features. This has important implications for methods that attempt to predict protein structure by "threading" techniques . Copley, R. R. and Barton, G. J. (1994), "A structural analysis of phosphate and sulphate binding sites in proteins: Estimation of propensities for binding and conservation of phosphate binding sites", J. Mol. Biol. , 242 , 321-329. Roberts, S., Ashmole, I., Gibson, L. J., Rookes, S. M., Barton, G. J. and Gallimore, P. H., (1994) "A mutational analysis of human papillomavirus E4 proteins: Identification of structural features important in the formation of cytoplasmic E4/cytokeratin networks in epithelial cells", J. Virol, 68,6432-6445. In this collaboration, our techniques for sequence comparison provided a link between E4 and actin.
Matthews, S., Barlow, P., Boyd, J., Barton, G. Russell, R., Mills, H., Cunningham, M., Meyers, N., Burns, N., Clark, N., Kingsman, S., Kingsman, A. and Campbell, I., (1994) "Structural similarity between the p17 matrix protein of HIV-1 and interferon-gamma", Nature, 370, 666-668. Our contribution to this collaboration was the discovery of structural similarity between p17 and interferon gamma. You can see an image of the superposition here (55k .gif). The p17 structure is shown in yellow, rabbit interferon in blue. You can also look here for more pictures and the abstract of the paper, or here for the two structures and a sequence alignment. Livingstone, C. D. and Barton, G. J. (1994), "Secondary Structure Prediction from Multiple Sequence Data: Blood Clotting Factor XIII and Yersinia Protein Tyrosine Phosphatase", Int. J. Pep. Prot. Res., 44, 239. The preprint of this paper is available as a PostScript document on our FTP server. You can link to the paper from here. You can also obtain Figure 1 and Figure 2 in the same way. Additional data too large to show in the printed paper is also available on this server. After submission of this paper, the tertiary structure of Protein Tyrosine Phosphatase 1B was determined by X-ray crystallography (Barford et al, 1994, Science, 263, 1397-1404). The prediction and X-ray structure agree well. You can see an image of the X-ray structre for PTPase with our secondary structure prediction shown in Red for predicted helix and blue for predicted strand, here (99k .gif). Barton, G. J., Cohen, P. T. C. and Barford, D. (1994), "Conservation Analysis and Structure Prediction of the Protein Serine/Threonine Phosphatases: Sequence Similarity with Diadenosine Tetra-phosphatase fromE. coli Suggests Homology to the Protein Phosphatases", Eur. J. Biochem.,220, 225-237. Gzip compressed PostScript version of this paper available here.
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